/ ANTIBODIES/ANTISERA / Carrier-Free Antibodies

Carrier-Free Antibodies

Carrier-free antibodies are particularly useful in applications where the presence of additional proteins can interfere with the assay or experiment. Fortis Life Sciences offers a subset of the Bethyl antibody portfolio in BSA-free format.

Antibody Conjugation and Labeling

Carrier-free antibodies are crucial for antibody conjugation and antibody labeling without requiring buffer exchange. These antibodies are compatible with a variety of conjugates and conjugation chemistries including fluorophores, enzymes, and epitope tags.

Immunoassays

The presence of BSA in antibody formulations can increase the signal-to-noise ratio and decrease the sensitivity of immunoassays by interfering with antibody binding to its intended target. Carrier-free antibodies eliminate the possibility of off-target antibody binding to BSA, improving assay sensitivity and specificity. Immunoassays that benefit from the use of carrier-free antibodies include:

  • Immunofluorescence/immunohistochemistry
  • Immunoprecipitation
  • Mass cytometry – CyTOF, imaging mass cytometry

Learn how BSA-free antibodies were used to develop an easy and adaptable system for generating conjugated antibodies to use in multiplex assays.
Poster: Epitope Tag Conjugation System Provides Versatility for Multiplex Assay Design

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